|Year : 2018 | Volume
| Issue : 2 | Page : 73-78
Estimation of insulin-like growth factor-1 as a biomarker in nonalcoholic fatty liver disease in patients with metabolic syndrome
Amal E El-Maghraby1, Eman A Aissa2, Hoda El H. Ahmed2
1 Department of General Medicine, Benha Teaching Hospital, Benha, Egypt
2 Department of Clinical Pathology, Benha Teaching Hospital, Benha, Egypt
|Date of Web Publication||28-Aug-2018|
Amal E El-Maghraby
Consultant of Internal Medicine, Benha Teaching Hospital, Benha
Source of Support: None, Conflict of Interest: None
Nonalcoholic fatty liver disease (NAFLD) is becoming the most common chronic liver pathology especially in developed countries, ranging from simple liver steatosis to nonalcoholic steatohepatitis. Patients with NAFLD having metabolic syndrome have abnormal liver enzymes that positively correlate with the number of metabolic syndrome components. Abdominal ultrasonography is frequently used in epidemiological studies to detect NAFLD. Insulin-like growth factor (IGF-1) is the critical hormone in the pathophysiology of metabolic syndrome and is associated with cardiovascular disease, diabetes mellitus, and abnormal lipid metabolism.
Participants and methods
A total of 70 patients, 50 patients having NAFLD by ultrasonography and 20 individuals as control, matched for age, sex, BMI, and waist circumference were clinically assessed. Abdominal ultrasonography was done, and laboratory investigations included estimation of IGF-1, homeostasis model assessment insulin resistance (HOMA IR), blood glucose, alanine aminotransferase (ALT), and lipid profile.
IGF-1 is highly significantly lower in the patient group than in the control group (P < 0.01). Moreover, there was a highly significant correlation between the low concentration of IGF-1 and BMI, waist circumference, fasting blood glucose, homeostasis model assessment insulin resistance, ALT, and serum cholesterol in the patient group (P < 0.01) and insignificant correlation with serum triglyceride and serum insulin (P > 0.05).
IGF-1 is lower in patients with NAFLD, especially those with elevated ALT, and is associated with increased number of metabolic syndrome components. Improving lifestyle, by decreasing body weight and controlling blood sugar using insulin sensitizers like pioglitazone, GLP-1 inhibitors like liraglutide, and SLGT2 inhibitors, and improving lipid profile are associated with good prognosis of NAFLD.
Keywords: IGF-1, liver disease, metabolic syndrome
|How to cite this article:|
El-Maghraby AE, Aissa EA, El H. Ahmed H. Estimation of insulin-like growth factor-1 as a biomarker in nonalcoholic fatty liver disease in patients with metabolic syndrome. J Med Sci Res 2018;1:73-8
|How to cite this URL:|
El-Maghraby AE, Aissa EA, El H. Ahmed H. Estimation of insulin-like growth factor-1 as a biomarker in nonalcoholic fatty liver disease in patients with metabolic syndrome. J Med Sci Res [serial online] 2018 [cited 2020 Feb 18];1:73-8. Available from: http://www.jmsr.eg.net/text.asp?2018/1/2/73/239801
| Introduction|| |
Central obesity is associated with many cardiometabolic risk factors like nonalcoholic fatty liver disease (NAFLD), which is associated with type II diabetes mellitus, insulin resistance, and dyslipidemia. Hence, it is considered to be the hepatic manifestation of metabolic syndrome.
NAFLD is becoming the most common chronic liver pathology especially in developed countries in both adults and children and ranges from simple liver steatosis to nonalcoholic steatohepatitis (NASH), which later progresses to advanced liver cirrhosis and end-stage liver disease .
However, most of the patients with NAFLD with metabolic syndrome have abnormal liver enzymes that positively correlate with the number of metabolic syndrome components, which suggests that the more the number of metabolic syndrome components, the higher the incidence of the elevated liver enzymes .
NAFLD is defined as the accumulation of fat in the liver exceeding 5% of its weight without significant alcohol intake; progression to steatohepatitis (NASH) occurs in up to 20% of patients with NAFLD, and until now why these percentages of patients develop NASH is not well understood .
Abdominal ultrasonography is frequently used in epidemiological studies to detect NAFLD. Detection of steatosis is only estimated when the hepatic fat content exceeds 33% on liver biopsy, so steatomarkers may be used like alanine aminotransferase (ALT) level and γ-glutamyl transferases .
Insulin-like growth factor (IGF-1) is considered the critical hormone in the pathophysiology of metabolic syndrome. The liver mainly produces it, and growth hormone stimulates its secretion, and its deficit is associated with cardiovascular disease, diabetes mellitus, and abnormal lipid metabolism .
IGF-1 decreases in NAFLD owing to hepatic insulin resistance modulate growth hormone-stimulated synthesis of hepatic (IGF-1) .
IGF-1 is responsible for increasing insulin resistance, impaired insulin metabolism, aggravating oxidative damage, and deregulating the neurohormonal axis, and there is an inverse relationship between IGF-1 levels and the metabolic syndrome-associated cardiovascular complications .
| Participants and Methods|| |
The study included seventy participants, comprising 50 patients diagnosed with NAFLD by abdominal ultrasonography and 20 healthy persons as a control group. They were selected from Internal Medicine Outpatient Clinic of Benha Teaching Hospital.
There were 15 male and 35 female patients, with age ranged from 30 to 60 years. The patients had at least one risk factor of metabolic syndrome (elevated blood pressure and dyslipidemia, dysglycemia, overweight, obesity, and family history of diabetes or cardiovascular disease); none of them were hepatitis C or B positive; none of them took drugs like corticosteroids, antidepressants, cordarone, or methotrexate; and none of the females were pregnant. The control group was composed of healthy persons, with 10 male and 10 female individuals. All participants were subjected to clinical examination, measurement of blood pressure, estimation of BMI, waist circumference, and abdominal ultrasonography.
Laboratory studies were also done, including serum blood glucose, lipid profile, ALT, estimation of IGF-1 and serum insulin, and calculation of homeostasis model assessment insulin resistance (HOMA IR).
A volume of 5 ml of venous blood through venipuncture was obtained in a plain tube without anticoagulant from patients fasting for 12 h. Samples are allowed to clot for 30 min at 37°C before centrifugation for 20 min at 1000g. Separated serum was divided for measurement of total cholesterol, triglyceride, ALT, and 1 ml was aliquoted and stored at −20°C for measurement insulin and IGF-1.
- Serum cholesterol was measured by enzymatic colorimetric cholesterol oxidase method using kits from Spinreact (SAU), Ctra. Sta. Coloma, 7 17176 St. Esteve de Bas GIRONA - Spain
- Serum triglyceride was measured by enzymatic colorimetric lipoprotein lipase method using kits from Spinreact
- Blood glucose level was measured by enzymatic colorimetric glucose oxidase method by using kits from Spinreact
- ALT was measured by kinetic ultraviolet method by kits from Centronic GmbH (Germany) 
- Enzyme-linked immunosorbent assay measured insulin using ELISA technique by commercial kit available from Immunospec Corporation (Canoga Park, California, USA) 
- Serum IGF-1 measurements were done by ELISA technique using Mybiosource kits (MyBioSource, Inc., San Diego, CA 92195-3308, USA) .
The principle for the measurment of insulin and IGF-1.
The assay system utilizes anti-insulin or anti-IGF-1 antibody for solid phase immobilization and another anti-insulin or IGF-1 antibody in the antibody–enzyme (horseradish peroxidase) conjugate solution. If human insulin or IGF-1 is present in the specimen, it will combine with the antibody on the well and the enzyme conjugate, resulting in the insulin or IGF-1 molecules being sandwiched between the solid phase and enzyme-linked antibodies.
A solution of tetramethylbenzidine was added and incubated, resulting in development of a blue color. The concentration of insulin or IGF-1 is directly proportional to the color intensity of the test sample.
Homeostasis model assessment insulin resistance
Homeostatic model assessment/insulin resistance  is a method for assessing B-cell function (% B) and insulin sensitivity (%S) through a computer model (HOMA 2): fasting insulin × fasting glucose/405.
It is used to assess the risk of developing diabetes and to assess response to treatment and is categorized as follows:
- Normal insulin resistance HOMA score less than 3
- Moderate insulin resistance between 3 and 5
- Severe insulin resistance greater than 5.
The collected data were tabulated and analyzed using SPSS software version 14 (SPSS Inc., Chicago, Illinois, USA). Data were expressed as χ2, mean ± SD, SD, analysis of variance, and Pearson's correlation studies.
| Results|| |
Our study included 70 participants. There were 50 patients with NAFLD, diagnosed by abdominal ultrasonography and had at least one risk factor for metabolic syndrome [15 (30%) males and 35 (70%) females], and 20 healthy participants [10 (50%) males and 10 (50%) females]. Their age ranged from 30 to 60 years [Table 1].
The comparison between patients group and control group showed the following.
Serum insulin was higher in the patient group in contrast to the control group (mean ± SD: 24.0 ± 3.4 vs. 15.1 ± 3.2) and was highly significant (P < 0.01). HOMA IR was also higher in the patient group compared with the control group (mean ± SD: 9.1 ± 1.9 vs. 2.9 ± 0.7) and the difference was highly significant (P < 0.01; [Table 2]).
IGF-1 was lower in the patient group in comparison with the control group (mean ± SD: 286 ± 14.3 vs. 340.5 ± 12.6), and also the relationship was statistically significant (P < 0.01; [Table 2], [Figure 1]).
|Figure 1: Insulin-like growth factor-1 mean in the patient and the control groups.|
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ALT showed higher concentration in the patient group in comparison with the control group (mean ± SD: 73.2 ± 21.9 vs. 16.6 ± 4.7), and the difference was highly significant (P < 0.01; [Table 2]).
There was also a highly significant correlation between fasting blood glucose (FBG), BMI, waist circumference, serum cholesterol, and serum triglycerides between the patient group and the control group (P < 0.01; [Table 2]).
The correlation between IGF-1 and different variables showed the following: there was a significant correlation between the lower concentration of IGF-1 and BMI and waist circumference in the patient group (P < 0.05), and this correlation was insignificant in the control group (P > 0.05; [Table 3], [Figure 2]).
|Table 3: Correlation between insulin-like growth factor-1 and other variables in patient and control groups|
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Moreover, the correlation between IGF-1 and FBG, HOMA IR, ALT, and serum cholesterol was highly significant in the patient group (P < 0.01) whereas insignificant in the control group (P > 0.05). However, the correlation between IGF-1 and serum triglyceride and serum insulin was negligible in both patient and control groups (P > 0.05; [Table 3], [Figure 3] and [Figure 4]).
|Figure 3: Relationship of insulin-like growth factor-1 and homeostasis model assessment insulin resistance.|
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|Figure 4: Correlation of insulin-like growth factor-1 and alanine aminotransferase.|
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| Discussion|| |
NAFLD is the most common liver condition in the world. The prevalence is up to 30% in developed countries and ~10% in the developing countries. It is caused by accumulation of triglyceride in the hepatocytes. Patients with NAFLD are also liable to increased risk of cardiovascular diseases, diabetes, hepatocellular injury, inflammation, and cirrhosis ,.
NAFLD is the hepatic manifestation of metabolic syndrome and is associated with obesity, insulin resistance, and independent predictor of type II diabetes mellitus . IGF-1 is a protein encoded by IGF-1 gene. It is similar in molecular structure to insulin and plays a vital role in childhood growth and has an anabolic effect in adults. IGF-1 consists of seventy amino acids in a single chain with three intramolecular disulfide brides .
The liver produces IGF-1 as an endocrine hormone stimulated by growth hormone; it is created throughout life, but the highest rates of IGF-1 production occur during the pubertal growth and the lowest levels in infancy and old age .
NAFLD has been described in adults with growth hormone-deficiency syndrome, and chronic liver disease can be associated with significant changes of levels of IGF-1, GF-binding proteins and insulin growth factor-binding proteins and acid labile subunits ,.
In epidemiological studies, ultrasonography has been extensively used to detect NAFLD and detect steatosis when fat content of hepatocytes exceed 33% on liver biopsy. Moreover, steatomarkers are used such as ALT and γ-glutamyl transferase .
Exploring the relation between metabolic risk factors related to type II diabetes mellitus and development of NAFLD, a study was done on 185 patient with type II diabetes and NAFLD versus 204 cases of type II diabetes alone. The study reported that postprandial blood glucose and insulin as well as BMI and HOMA IR were higher in patients with type II diabetes with NAFLD . Moreover, Savastano et al.  demonstrated that HOMA IR, ALT, and CRP were significantly higher in patients with severe hepatic steatosis than in those with mild hepatic steatosis. This is concomitant with a study on 115 obese patients with BMI greater than 30 kg/m2 (65 patients with liver steatosis and 50 controls), where HOMA IR, serum insulin, and blood glucose were significantly higher in patients than in control . These results came in agreement with our study, which shows a highly significant correlation between FBG, serum insulin, and HOMA IR in the patient group with metabolic syndrome and NAFLD in comparison with the control group (P < 0.01).
Waist circumference is a risk factor for metabolic syndrome and is related to insulin resistance and elevated ALT in patients with NAFLD, which was significantly associated with an increase in waist circumference .
In a Brazilian study, both BMI and waist circumference were associated with increased steatohepatitis with liver fibrosis on liver biopsy . However, Graffigha et al. , demonstrate also a high prevalence of NAFLD in patients who were overweight or obese, and hepatic steatosis detected by abdominal ultrasonography was positively correlated with waist circumference and serum triglyceride. This was in agreement with our study, which shows a highly significant correlation of BMI, waist circumference, serum triglycerides, and serum cholesterol in the patient group in comparison with the control group (P < 0.01).
Most patients with NAFLD have abnormal liver enzymes and higher in patients with metabolic syndrome. Liver enzyme levels significantly increase with the number of metabolic syndrome components .
Leitc et al. , demonstrated that low adiponectin and low transforming growth factor (IGF-B1) are associated with severe NAFLD stages in type II diabetes, and a combination of HOMA and adiponectin to hepatic leptin ratio and ALT ratio may be a useful noninvasive technique to determine the severity of liver damage ,. Cruz et al. , also found that ALT and HOMA IR are correlated with the degrees of hepatic steatosis on ultrasonography and can help in the selection of patients for liver histological evaluation.
In a study was done on 235 patients with biopsy-proven NAFLD, patients with NAFLD and diabetes mellitus were significantly older, predominantly females, had significantly higher AST/ALT ratio and FIB-4 index, and have higher fibrosis score compared with nondiabetic ones .
Moreover, in a study done on 141 people with diabetes with fatty liver detected by abdominal ultrasonography showing increased hepatic echogenicity and vascular blunting, where liver stiffness measurement greater than or equal to 7 kPa represented hepatic fibrosis, it was found that 16% of diabetic with NAFLD have significant hepatic fibrosis and significant elevated ALT .
Another study on 52 patients of nondiabetic NAFLD with hyperechogenicity of the liver by ultrasonography found HOMA insulin resistance was significantly related to hepatomegaly and ALT increase, which was present in 36% of patients .
Those studies were concomitant with our research, as there was a highly significant correlation of ALT and HOMA IR in the patient group with NAFLD than in the control group (P < 0.01).
Insulin growth factor deficiency is associated with deregulated lipid metabolism, cardiovascular disease, diabetics, and altered metabolic profile in diabetics, and also it is responsible for stimulation of protein synthesis in the muscle, lowering glucose level in type I and II diabetes mellitus by enhancing insulin sensitivity ,.
The liver mainly produces serum IGF-1 and its deficiency is a determinant of metabolic syndrome like insulin resistance, abnormal lipid profile, type II diabetes mellitus, and increased waist–hip ratio, and growing risk of fatal coronary events .
In a study by Colak et al. , IGF-1 level was significantly decreased in patients with moderate to severe fibrosis and proved that IGF-1 might be useful to differentiate advanced fibrosis in patients with NAFLD.
In another Japanese study on 199 patients with biopsy-proven NAFLD, serum IGF-1 was significantly lower in patients with NASH compared to NAFLD with increasing lobular inflammation .
These studies are in agreement with our study, as IGF-1 was considerably lower in the patient group compared with the control group.
Moreover, ALT and IGF-1 level were significantly associated with differences in fibrosis . Graffigha et al. , also found that the ultrasonographic features of steatosis were correlated positively with waist circumference, serum triglyceride, serum insulin, and HOMA IR and negatively correlated with IGF-1.
In our study, lower level of IGF-1 is significantly associated with BMI and waist circumference (P < 0.05) and highly significant correlation with FBG, HOMA IR, ALT, and serum cholesterol (P < 0.01) but insignificantly correlated with serum triglyceride and serum insulin (P > 0.05).
Lifestyle usually improves insulin sensitivity and hepatic steatosis, and weight loss by about 10% can significantly reverse hepatocyte injury, enhancing bariatric surgery can also achieve NASH .
The use of insulin sensitizers like pioglitazone, especially with type II diabetes mellitus or impaired glucose tolerance, can decrease hepatic steatosis and reduce necroinflammation and hepatic fibrosis .
However, a recent meta-analysis of clinical trials of liraglutide in type II diabetes mellitus has suggested that glucagon-like peptide-1 receptor agonist could improve NASH; in contrast, studies with dipeptidyl peptidase (DPP4-inhibitors) have reported mixed results. Sodium-glucose co-transporter 2(SGLT2 inhibitors) have antifibrotic properties on animal models with NASH and in patients treated with SGLT2, and ALT decreases during treatment .
| Conclusion|| |
Estimation of IGF-1 is a critical noninvasive biomarker in patients with NASH with metabolic syndrome; its decrease is associated with cardiovascular disease, diabetes mellitus, and abnormal lipid metabolism. The lower the serum IGF-1, the more the liability to metabolic and cardiovascular complications and hepatic complications like steatohepatitis and hepatic fibrosis.
Improving patients' lifestyle, decreasing body weight, and controlling lipid profile and blood sugar in people with diabetes using insulin sensitizers like pioglitazone, GLP-1 inhibitors like liraglutide, and SGLT2 inhibitors have prognostic value in improving patients with NASH.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Lebensztejn DM, Flisiak-Jackieweicz M, Bialokoz-Kalinowsk I, Bobrus-Chociej A, Kowalska J. Hepatokines and non-alcoholic fatty liver disease. Acta Biochem Pol 2016; 63:459–467.
Liu M, Yan HM, Gao X, Gao J. Association of abnormality of liver enzymes and metabolic syndrome in patients with non-alcoholic fatty liver disease. Zhonghya Yi Xue Zazhi 2007; 87:253–255.
Jahn D, Rau M, Wohlfahrt Y, Hermanns HN, Geier A. Non alcoholic steatohepatitis: from pathophysiology to novel therapy. Dig Dis 2016; 34:346–363.
Saadeh S, Younossiz M, Remer EM, Gramlich T, Ongy P, Hurley M, et al
. The utility of radiological imaging in nonalcoholic fatty liver disease. Gastroenterology 2002; 123:745–750.
Aguirre GA, DeItei JR, Dela Garzo RG, Castillo-Cortozor I. Insulin-like growth factor-1 deficiency and metabolic syndrome. J Tranl Med 2016; 14:3.
Arturi F, Succurro E, Procopio L, Pedag E, Mannino GG, Lugara M, et al
. Nonalcoholic fatty liver disease is associated with low circulating levels of insulin-like growth factor-1. J Clin Endocrinol Metab 2011; 96:E1640–E1644.
De Ita JR, Castilla Cortazar I, Aguirre GA, Sanche Z, Yago C, Santos-Ruiz MO, et al
. Altered liver expression f
genes involved in lipid and glucose metabolism in mice with partial IGF-1 deficiency: an experimental approach to metabolic syndrome. J Tranl Med 2016; 14:3.
Prati D, Taioli E, Zanella A, Torre ED, Butelli S, Vecchio ED, et al
. Updated definitions of healthy ranges for serum alanine aminotransferase levels. Ann Intern Med 2002; 137:1–9.
Boehm TM, Lebovitz HE. Statistical analysis of glucose and insulin responses to intravenous tolbutamide: evaluation of hyperglycemic and hyperinsulinemic states. Diabetes Care 1979; 6:479–490.
Aleman A, Verhaar HJ, de Haan EH, de Vries WR, Samsom MM, Drent ML, et al
. Insulin like growth factor-1 and cognitive function in healthy older men. J Clin Endocr Metab 1999; 84:471–475.
GayosoDiz P, Otero-González A, Rodriguez-Alvarez MX, Gude F, García F, De Francisco A, González Quintela A. Insulin resistance (HOMA-IR) cut-off values and the metabolic syndrome in a general adult population: effect of gender and age: EPIRCE cross-sectional study. BMC Endocr Disord 2013; 13:47.
Smith BW, Adam LA. Nonalcoholic fatty liver disease. Cret Rev Clin Lab Sci 2011; 48:97–113.
Cusi K. Treatment with type II diabetes and nonalcoholic fatty liver disease: current approaches and future directions. Diabetologia 2016; 59:1112–1120.
Kotronen A, YkiYorvinen H. Fatty liver: a novel component of metabolic syndrome. Arterio Scler Thromb Vasc Biol 2008; 28:27–28.
Girnito L, Worrall C, Tkahashi S, Seregard S, Girnia A. Something old, something new and soothing borrowed: emerging paradigm of insulin-like growth factor type-1 receptor (IGF-IR) signaling regulation cellular and molecular life. Science 2014; 1:2403–2427.
Gallagher EJ, Le Roith D. is growth hormone resistance (IGF-1) reduction good for you? Cell Metab 2011; 13:355–356.
Fusco A, Miclo L, D'Uonnolo A, Forgione A, Riccardi L, Cefalo C, et al
. Nonalcoholic fatty liver disease is associated with increased GH BP and reduce GH/IGF-1 level. Clin Endocrinol (Ocf) 2012; 77:531–536.
Chishima S, Kogiso T, Matsushita N, Hashimoto F, Tokusuge K. The relationship between the growth hormone/insulin-like growth factor system and the histological features of non-alcoholic fatty live disease. Intern Med 2017; 56:473–480.
Guo M, Xi G, Yang G, Yao H. Analysis of metabolic risk factors for developing nonalcoholic fatty live in patients with type II diabetes mellitus. Zhonghua Gan Zang Bing Za Zhi 2014; 22:631–635.
Savastano S, Di Somma C, Pizza G, De Rosa A, Nedi V, Rossi A, et al
. Liver-spleen axis, insulin-likegrowth factor (IGF-1) axis and fat mass in overweight-obese females. J Trans Med 2011; 9:136.
Ju DY, Choe YG, Cho YK, Shin DS, Yoo SH, Yim SH, et al
. The influence of waist circumference on insulin resistance and nonalcoholic fatty liver disease in apparently healthy Korean adults. Clin Mol Hepatol 2013; 19:140–147.
Rocha R, Cotrim HP, Carvalho FM, Siqueira AC, Braga H, Freitas LA. Body mass index and waist circumference in nonalcoholic fatty liver disease. J Hum Nutr Doct 2005; 18:365–370.
Graffigha MN, Belli SH, De Larranaga G, Fainboim H, Estepo C, Pres S, et al
. Insulin-like growth factor binding protein-1: a new biomarker of nonalcoholic fatty liver disease. Acta Gastroenterol Latinoam 2009; 39:30–37.
Leitc NC, Salles GF, Cardoso CR, Villela-Noguerira CA. ca. Serum biomarkers in type II diabetic patients with non-alcoholic steatohepatitis and advanced fibrosis. Hepatol Res 2013; 43:508–515.
Lemoine M, Ratziu V, Kim M, Maachi M, Wendum D, Pay EF, et al
. Serum adipokine levels predictive of liver injury in nonalcoholic fatty liver disease. Worl J Hepatol 2015; 7:2610–2618.
Cruz MA, Cruz JF, Maceno LB, De Santana DS, Olivera CE, Lima SO, et al
. Association of the nonalcoholic hepatic steatosis and its degrees with the values of liver enzymes and homeostasis model assessment – insulin resistance index. Gastroenterology Res 2015; 8:260–264.
Puchakalaya BK, Verma S, Kanwar P, Hart J, Sanivarapu RR, Mohnty SR. Histopathological difference utilizing the nonalcoholic fatty liver disease activity score criteria in diabetic type II diabetes and nondiabetic patients with non alcoholic fatty liver disease. World J Hepatol 2015; 7:2610–2618.
Sabhonslidsuk A, Pulsombat A, Kalwdoung P, Petraksa S. Non alcoholic fatty liver disease (NAFLD) and significant hepatic fibrosis defined by noninvasive assessment in patients with type II diabetes. Asi An Pac J Cancer Prev 2015; 16:1789–1794.
Cankurtaran M, Tayfur O, Yavuz B, Geyik S, Akhan O, Arslan S. Insulin resistance and metabolic syndrome in patients with NAFLD but without diabetes. Effect of a 6-month regimen intervention. Acta Gastroenterol Belg 2007; 70:253–259.
Clemmons DR. Metabolic actions of insulin-like growth factor-1 in normal physiology and diabetes. Endocrinol Metab Clin North Am 2012; 41:425–443.
Succuro E, Andereozzi F, Marini MA, Lauro R, Hribal ML, Pertivune F, Sesti G. Low plasma insulin-like growth factor-1 levels are associated with reduced insulin sensitivity and increased insulin secretion in nondiabetic subjects. Nutri Metab Cardiovasc Dis 2009; 19:713–719.
Colak Y, Senates E, Ozturk O, Yilmoz Y, Zemheri E, Yilmaz E, et al
. Serum concentrations of human insulin-like growth factor-1and levels of insulin-like growth factor-binding protein-5 in patients with nonalcoholic fatty liver disease: association with liver histology. Eur J Gastroenterol Hepatol 2012; 24:255–261.
Sumida Y, Senates E, Oztwk O, Yilmoz Y, Zenheri E, Yilma Z, et al
. Lower levels of insulin-like growth factor-1 standard deviation score are associated with histological severity of the non-alcoholic fatty liver disease. Hepatol Res 2015; 45:771–781.
Ichikawa T, Nakao K, Hanasaki K, Furukawa R, Tsuruta S, Veda Y, et al
. Role of growth hormone, insulin-like growth factor-1 and insulin-like growth factor-binding protein 3 in development of the non-alcoholic fatty liver disease. Hepatol Int 2007; 1:287–294.
Lomonaco R, Sunny NE, Bril F, Cust K. Non alcoholic fatty liver disease. Current issues and novel treatment approaches. Drugs 2013; 73:1–14.
Chang Y, Jung HS, Yun KE, Cho J, Cho YK, Ryu S. Cohort study of non-alcoholic fatty liver disease. NAFLD fibrosis score and incident diabetes in a Korean population. Am J Gastroenterol 2013; 108:1861–1868.
Sumida Y, Yoneda M. Current and future pharmacological therapies for NAFLD/NASH. J Gastroenterol 2018; 53:362–376.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2], [Table 3]