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Table of Contents
ORIGINAL ARTICLE
Year : 2019  |  Volume : 2  |  Issue : 4  |  Page : 257-264

Glutamic acid decarboxylase auto-antibodies prevalence among patients with type 2 diabetes mellitus and their clinical characteristics in a sample of the Egyptian population


1 Department of Clinical and Chemical Pathology; Department of Internal Medicine, National Institute of Diabetes and Endocrinology, Cairo, Egypt
2 Department of Clinical and Chemical Pathology; Department of Internal Medicine, National Institute of Diabetes and Endocrinology, Cairo, Egypt

Date of Web Publication10-Feb-2020

Correspondence Address:
Ayat I Ghanem
5 Amin El Rafeey Street, Dokki 12611
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JMISR.JMISR_49_19

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  Abstract 


Background
Patients with latent autoimmune diabetes in adults may develop early loss of β-cell mass. They are often misdiagnosed as having type 2 diabetes mellitus (T2DM). This is because latent autoimmune diabetes in adults condition does share characteristics of both type 1 and T2DM.
Aim
To estimate the prevalence of unrecognized cases with glutamic acid decarboxylase auto-antibodies (GADA) among patients with T2DM in a sample of the Egyptian population, describing their clinical and laboratory features, and investigating the relationship with chronic complications of diabetes.
Settings and design
A total of 1515 Egyptian patients previously diagnosed as having T2DM were divided into two groups based on presence versus absence of GADA. Demographic and clinical characteristics were compared.
Patients and methods
Patients were selectively admitted according to clear criteria after informed consent and approval of the Ethics Committee. Patients underwent comprehensive history taking and examination. Laboratory investigations included blood chemistry, C-peptide, and GADA.
Statistical analysis
Data were analyzed using SPSS.
Results
Overall, 12.8% of patients with T2DM were GADA positive, showing lower C-peptide levels (P < 0.001). GADA-positive patients with T2DM demonstrated a positive correlation of GADA levels with central obesity (r = 0.175; P = 0.015) and systolic blood pressure (r = 0.171; P = 0.018). Moreover, a negative correlation with cardiovascular diseases (r=−0.0175; P = 0.015) was observed.
Conclusion
Prevalence of GADA among patients with T2DM in the analyzed sample was 12.8%, which agrees with the findings in other populations. These patients had lower C-peptide levels. GADA measurements are recommended in patients with T2DM with low C-peptide levels, especially in newly diagnosed patients, to help identify positive cases and initiate early insulin treatment to help preserve β-cell function and decrease the risk of long-term complications.

Keywords: Glutamic acid decarboxylase auto-antibodies, latent autoimmune diabetes in adults, type 2 diabetes mellitus


How to cite this article:
Ghanem AI, Bassyounia AA, Omar GA. Glutamic acid decarboxylase auto-antibodies prevalence among patients with type 2 diabetes mellitus and their clinical characteristics in a sample of the Egyptian population. J Med Sci Res 2019;2:257-64

How to cite this URL:
Ghanem AI, Bassyounia AA, Omar GA. Glutamic acid decarboxylase auto-antibodies prevalence among patients with type 2 diabetes mellitus and their clinical characteristics in a sample of the Egyptian population. J Med Sci Res [serial online] 2019 [cited 2020 Apr 6];2:257-64. Available from: http://www.jmsr.eg.net/text.asp?2019/2/4/257/278065




  Introduction Top


Diabetes is one of the most common metabolic disorders in many developing countries and in most developed countries[1]. The International Diabetes Federation has identified Egypt as the ninth leading country in the world for the number of patients with type 2 diabetes mellitus (T2DM). Its prevalence in Egypt has almost tripled over the past two decades[2].

Diabetes is a complex disease, and type 1 and type 2 clinical classification does not capture the range of diseases included in the diagnosis[3]. The common form of T2DM is a combination of insulin resistance and progressive loss of β-cell function. On the contrary, type 1 diabetes mellitus (T1DM) is usually the result of complete or near-complete destruction of β-cells mediated by autoimmunity. Latent autoimmune diabetes in adults (LADA), a less recognized and underdiagnosed manifestation of diabetes mellitus (DM), appears to affect adults with many T2DM characteristics but with a high risk of progression of insulin dependence[4]. Although the American Diabetes Association and WHO do not recognize LADA as a separate entity, it is included within T1DM classification[5],[6]. LADA-affected patients are often misdiagnosed and treated as patients with T2DM[7].

Three main diagnostic criteria for LADA that have been established by the Immunology of Diabetes Society, include the following: (a) adult age of onset (>30 years), (b) any autoantibody islet cell presence, and (c) no insulin requirement for at least 6 months after the diagnosis[8]. Currently, glutamic acid decarboxylase auto-antibodies (GADA) is thought to be the most sensitive and specific biomarker in the diagnosis of LADA[9]. Being the most frequent islet autoantibody, many studies recommended that detection of GADA alone is sufficient when screening for patients with LADA, and that measurement of other islet auto-antibodies are not advisable[10]. Multicenter studies in Europe, North America, and Asia showed that 4–14% of patients with T2DM were positive for islet cell auto-antibodies that were diagnosed with LADA[11]. Furthermore, the prevalence of GADA-positive subgroup of adult patients initially diagnosed with T2DM together with additional criteria of no exogenous insulin during the first 6–12 months was 25% in patients under 35 years and between 4 and 13% in patients over 35 years at the diagnosis in populations of European origin[12].

As the prevalence of diabetes in Egypt has been observed to be remarkably high, the prevalence of LADA in this population is expected to be high, and very little work has been done in the context of LADA in the Egyptian population. The primary aim of this study was to estimate the prevalence of GADA positivity in Egyptian patients with T2DM and to describe their clinical and laboratory features and its relationship with chronic complications of diabetes.


  Patients and Methods Top


This study was carried out between 2016 and 2018 comprising 1515 Egyptian patients previously diagnosed as having T2DM. They comprised 1304 females and 211 males. They were divided into two groups based on presence versus absence of GADA. The study was approved by the responsible ethics committee. In addition, written informed consent was obtained from each participant, before their inclusion in the study.

Inclusion criteria

Inclusion criteria included patients with T2DM aged between 35 and 70 years old diagnosed according to the criteria of the American Diabetes Association[5]. They were receiving insulin treatment after an initial period of at least 6 months following the diagnosis of T2DM, during which noninsulin modalities of treatment had been sufficient to achieve adequate glycemic control.

Exclusion criteria

Exclusion criteria were patients with T1DM, known history of thyroid disease, pregnancy, unstable cardiac disease, renal impairment, liver cirrhosis, and malignancies.

All patients were subjected to proper history taking, including duration of DM, current treatment, family history of DM, and other comorbidities. Anthropometric measurements, included BMI, calculated as weight in kilograms/height in square meters, and waist circumference, a validated simple noninvasive central adiposity surrogate, which was measured as the midpoint between the lowest rib and the iliac crest.

They were all subjected to standard physical examination for chronic complications of diabetes and cardiovascular risk factors and diseases. The prevalence of hypertension was defined as systolic blood pressure (BP) of at least 140 mmHg and/or diastolic BP of at least 90 mmHg and/or current treatment with BP-lowering drug (s) prescribed for treating high BP. Peripheral neuropathy was achieved as positive when vibration sense by a 128-Hz vibration fork at both hallux was absent, combined with a disturbed 10 g Semmes–Weinstein monofilament test and absent ankle reflexes. The retinopathy diagnosis was based on fundoscopy by an ophthalmologist after pharmacological mydriasis. Nephropathy was diagnosed by moderately albumin excretion in urine (>30 mg albumin/24 h) by albumin/creatinine ratio estimation. Regarding macroangiopathy, coronary artery disease has been concluded retrospectively from medical history (myocardial infarction, angioplasty, stenting, revascularization and/or substantial angiographically confirmed coronary stenosis) and systematic evaluation of all procedures, screening (exercise testing, echocardiography), or subclinical disease imaging data in the patient's documentation. Peripheral artery disease has been identified in Doppler ultrasonography and/or angiography through a well-recorded medical history of lower-limb claudication and/or clinical or imaging evidence of ischemic diabetic foot, angioplasty, stenting, revascularization, and/or significant lower-limb artery stenosis. Stroke was defined according to the criteria of the UK Prospective Diabetes Study, that is, any neurological deficiency with symptoms or signs lasting for at least 1 month, with no diffidence made among ischemic, embolic, and hemorrhagic strokes.

Sample collection and laboratory analysis

From each patient, 8 ml of venous blood was withdrawn and divided into three sample tubes after (12–14 h) overnight fasting. First 4 ml was put in a serum separator tube for blood chemistry and was left to clot. Serum was rapidly separated by centrifugation at 3000 rpm for 10 min. It was tested for fasting blood glucose and lipid profile [total cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-c), and low density lipoprotein cholesterol] by using BT 3500, Clinical Chemistry, Turbidimetric and ISE analyzer (Rome, Italy; Alfa Diagnostic (Biotecnica Instruments, Rome, Italy). The second 2 ml was put in a separate EDTA vacutainer for glycated hemoglobin (HbA1c) analysis by HPLC technique using D-10 HPLC ion exchange chromatography (BioRad, Hercules, California USA); Afak el Mostakbal Company, Cairo, Egypt). The last 2 ml was put in a serum separator tube and was left to clot. Serum was separated after centrifugation for 15 min at 3000 rpm. Serum was stored at –80°C until analysis of GADA and C-peptide levels by commercially available enzyme-linked immunosorbent assay (ELISA) technique according to manufacturer's instructions. GADA levels were determined with a ELISA assay based on microplate wells coated with human recombinant glutamic acid decarboxylase isoform (DRG International Inc., Springfield, New Jersey USA); http://www.drg-international.com). GADA value were read as follows: negative less than 1.00 U/ml, positive more than 1.050 U/ml, and indeterminate 1.00–1.050 U/ml (borderline). Borderline samples were retested, and they still had borderline values, another sample was taken for reanalysis. The DRG C-peptide ELISA kit is a solid-phase ELISA, based on the principle of competitive binding for quantitative assay. A random urine sample was collected in a sterile cup for measurement of micro-albumin and creatinine (A/C) in urine and calculation of micro-A/C ratio by using ARCHI TECT 8000 chemistry analyzer (Abbott, USA; Al-Kamal Company, Cairo, Egypt).

Statistical analysis

Data were analyzed using the IBM program SPSS 20.0 Package (SPSS Inc., Chicago, Illinois, USA). Quantitative data were expressed as mean ± SD and compared using t-test when normally distributed, and as median and range using Mann–Whitney U-test when not normally distributed. Categorical data were represented as frequencies (%). The differences in frequencies of categorical parameters were analyzed by χ2-test. Correlations were done using Pearson's correlation coefficient test (r) or Spearman's coefficient. P values less than 0.05 were considered statistically significant.


  Results Top


This study was conducted on 1515 Egyptian patients with T2DM, with an age range of 35–70 years, attending the outpatient clinic during the study period; they included 1304 (86%) females and 211 (14%) males. Of these patients, 1321 were GADA negative, representing 87.2%, and 194 were GADA positive, representing 12.8%.

Demographic and laboratory characteristics of the GADA negative in comparison with GADA positive patients with T2DM are mentioned in [Table 1]. Regarding age, duration of diabetes, BMI, waist circumference, and diastolic BP, no statistically significant differences were observed between the two groups (P = 0.409, 0.235, 0.072, and 0.763, respectively). The systolic BP in the GADA-negative group was significantly higher than in the GADA-positive group (P = 0.022). Regarding fasting blood glucose, HbA1c, total cholesterol, TG, low-density lipoprotein, HDL, and A/C ratio, there was no statistical significant difference (P > 0.05), but a highly statistical significant difference between the two groups in C-peptide level was noted (P < 0.001).
Table 1: Demographic and laboratory characteristics of the glutamic acid decarboxylase auto-antibodies negative in comparison with glutamic acid decarboxylase auto-antibodies positive among type 2 diabetes mellitus patients

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Furthermore, according to sex, there was no statistically significant difference between females and males in GADA-negative and GADA-positive groups (P = 0.377). Moreover, regarding age (≥50 and <50 years), BMI (underweight, normal and overweight groups), and central obesity (normal and central obesity), there was no statistically significant differences between the two groups (P = 0.409, 0.539, and 0.371, respectively), as shown in [Table 2].
Table 2: Comparison between glutamic acid decarboxylase auto-antibodies negative and glutamic acid decarboxylase auto-antibodies positive among type 2 diabetes mellitus patients as regards different categories of demographic characteristics

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Moreover, [Table 3] showed no statistically significant differences between both groups regarding clinical presentations of diabetic microvascular and macrovascular complications [nephropathy, retinopathy, peripheral neuropathy, cardiovascular diseases (CVD), peripheral vascular diseases, and stroke (P > 0.05)].
Table 3: Comparison between glutamic acid decarboxylase auto-antibodies negative and glutamic acid decarboxylase auto-antibodies positive among type 2 diabetes mellitus patients as regards clinical presentation of diabetic complications

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Considering the correlation between GADA and demographic and laboratory data in GADA-positive patients with T2DM, no statistically significant correlation was observed between GADA and age, sex, duration of diabetes, BMI, and diastolic BP (P > 0.05), but a positive correlation was noted with central obesity (r = 0.175; P = 0.015) and systolic BP (r = 0.171; P = 0.018). Regarding laboratory results, no statistically significant correlation was observed (P > 0.05; [Table 4]).
Table 4: Correlation between glutamic acid decarboxylase auto-antibodies with demographic and laboratory characteristics among glutamic acid decarboxylase auto-antibodies positive type 2 diabetes mellitus patients

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More interesting, the correlation between GADA-positive patients with T2DM and the clinical presentations of diabetic microvascular and macrovascular complications revealed a negative correlation with CVD (r=−0.0175; P = 0.015; [Table 5]).
Table 5: Correlation analysis between glutamic acid decarboxylase auto-antibodies with clinical presentation of diabetic complications among glutamic acid decarboxylase auto-antibodies positive type 2 diabetes mellitus patients

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  Discussion Top


Although sufficient data are available on the prevalence of diabetes in the Middle East, little is known about the characteristics of autoimmune diabetes in this area[13]. Knowledge of pathophysiology and the characteristics of the disease is extremely important to implement general preventive strategies and to treat diabetes-affected patients correctly, so that safe and effective therapies are possible[14]. Data from randomized clinical trials demonstrated the importance of an early initiation of insulin therapy in LADA avoiding the use of secretagogues[15].

The main finding of this study is the prevalence of GADA-positive patients, representing 12.8% (194 of 1515 Egyptian patients with T2DM) of the patients enrolled in the study. This is within the range cited in similar studies in European countries, where ~4–14% of patients classified with T2DM have diabetes-associated auto-antibodies, and the GADA frequency is high in northern European studies (7–14% with decreasing prevalence as patient age increases)[16]. Results from the Norwegian Nord-Trondelag Health (HUNT) study showed that of 1049 patients with T2DM, 106 (10.1%) were positive for GADA[17]. Moreover, this prevalence was found to be comparable with other studies. In the UK Prospective Diabetes Study group (25), which measured GADA and ICA at the diagnosis of diabetes in a representative population of 3672 White patients with T2DM (aged between 25 and 65 years), 361 (9.8%) were positive[18]. In BOTNIA study in Finland, of 1122 patients with T2DM, only 104 (9.3%) were found to have those auto-antibodies[19]. In action LADA 7, they consecutively studied 6156 European diabetic patients attending clinics within 5 years of diagnosis (age range: 30–70 years), and 541 (8.8%) had GADA[11]. Moreover, LADA and CARDS reported that out of 2425 European patients with presumed T2DM, 173 patients (7.1%) had GADA[20]. Similarly, in Bulgarian Population, the prevalence of positive diabetes-associated auto-antibodies among patients diagnosed with T2DM is 10.16%, and GADA were the main positive autoantibody[21]. Interestingly, in Saudi Arabian patients with T2DM, 8 of 99 patients were GADA positive (8%)[22].

However, in Southern Europe, Asia, and North America, it appears to be lower (4–6%). In 2007, it was found that out of 4250 consecutive patients with T2DM, 4.5% had either GADA and/or IA-2As[12]. Moreover, in a 2010 study, the screening of 5568 patients with T2DM for GADA identified 276 (5%) LADA patients[23]; another study in North America and Europe demonstrated that of 4134 patients with T2DM, 174 (4.2%) had GADA[24]. In Asia, the LADA China study revealed that of 4880 patients with T2DM, 5.9% had GADA. Furthermore, within China, the prevalence was lower in the south than the north[25]. Similarly, in Japan, it was concluded that GADA was detected in 188 (3.8%) of the 4980 diabetic patients tested[26], whereas the prevalence in the Korean population ranged from 4.4 to 5.3%[27].

The global variance in the frequency of autoantibody positivity in patients with T2DM could be primarily owing to differences in the study design, selection criteria, study entry duration, ethnicity, and sensitivity and specificity of autoantibody assay. Furthermore, increasing T2DM prevalence in some populations could affect the frequency of autoantibody positivity in these patients[28]. In fact, the three criteria that are conventionally used to define LADA are nonspecific, namely, age at diagnosis, autoantibody positivity, and insulin treatment. Adult age definitions range from 15 to 30 years, up to 70 years. Autoantibody criteria are lacking in specificity even in the best laboratories because they are based on auto-antibodies associated with T1DM in childhood that lack 100% specificity[29]. The definition of positive autoantibody is not clear, and various cutoff points have been applied in various studies. Technically, it is possible to limit false positives by setting a higher cutoff or retesting positive results. In longitudinal studies, the autoantibody status changes over time[30]; moreover, most patients are positive for only one type of autoantibody, the loss of existing auto-antibodies and the development of others may be a different scenario. The fluctuating status of the autoantibody is still not fully understood, and the clinical significance of borderline positivity remains unsettled[31].

Regarding sex, in this study, no statistically significant difference was observed between females and males, which is not consistent with other studies that reported a higher female prevalence in the GADA-positive patient group [32–34]. When comparing between GADA-negative and GADA-positive patients with T2DM regarding demographic and laboratory characteristics, only systolic BP in the GADA-positive group was significantly lower than in the GADA-negative group (P = 0.022). Many studies identified that patients with LADA generally have a better metabolic profile than those with T2DM, with lower BMI, waist-to-hip ratios, BP, and triglyceride and higher HDL-c levels[11],[12],[25],[35], which is not in accordance with results of this study. Furthermore, considering the correlation between GADA and demographic and metabolic profile in GADA-positive patients with T2DM, a positive correlation was noted with central obesity and systolic BP. The study findings were consistent with the Swedish and Norwegian records of increased risk of autoimmune diabetes associated with overweight and obesity[36]. On the contrary, a negative correlation was observed in the Korean population between GADA levels and onset age, BMI, total cholesterol, triglycerides, and C-peptide levels, whereas HbA1c and HDL-c showed a positive correlation[27].

Regarding HbA1c, patients with autoimmune diabetes tend to have worse glycemic control despite increased use of insulin than patients with T2DM HbA1c (>6.9%)[20],[37]. The study results showed no statistically significant difference between HbA1c mean levels in GADA-positive and GADA-negative groups of patients. In agreement, the LADA China study reported that this difference was not evident[25]. Another study found that early insulin therapy does not appear to improve control[38]. This study regarding C-peptide level showed that it was significantly lower in the GADA-positive T2DM (P < 0.01). Similarly, in the HUNT study, GADA positivity was found to be associated with decreased C-peptide fasting in adult patients with noninsulin-requiring diabetes[17]. In Italian patients, an inverse relationship was reported between GADA and C-peptide levels[39]. The fact that patients with autoimmune diabetes require insulin treatment more frequently and earlier after diagnosis than those with GADA-negative T2DM may be explained by that the low-grade inflammation and typical of visceral adiposity in obese patients with genetic susceptibility to T2DM, could trigger a low-grade autoimmune process marked by auto-antibodies positivity, resulting in loss of function of β-cell and impairment of insulin secretion[25]. On the contrary, in two large studies, insulin secretion was similar in recently diagnosed patients with autoimmune diabetes and T2DM[24],[40]. However, data from studies with the available new, second-line antidiabetic drugs suggested that the loss of B-cell function could be postponed[41],[42].

Concerning chronic microvascular complications of diabetes, there was no difference in the prevalence of nephropathy, retinopathy, and neuropathy between GADA-positive and GADA-negative patients with T2DM in this study. Similar to these results, there were no differences in the prevalence of microvascular complications in South Korea[27]. The Fremantle Diabetes Study's analysis of longitudinal data showed less frequency of albumin excretion in urine in newly diagnosed patients with LADA than in patients with T2DM. In addition, a reduced risk of developing albumin excretion in urine during the follow-up period (<5 years) was associated with GADA positivity[43]. Another cross-sectional study confirmed that in Chinese patients with LADA, nephropathy and retinopathy rates were lower than in patients with T2DM[44]. This observation, however, was only evident for people with a disease duration of less than 5 years[45]. Accordingly, studies evaluating patients with T2DM with a duration of disease more than 5 years who were positive for GADA had equal or even higher rates of microvascular complications than those who were negative for GADA[20]. Furthermore, studies investigating diabetic neuropathy have shown that neuropathy is generally more prevalent in LADA than in T2DM[27],[45], except for a study by Baum et al.[46]. These findings, however, could be explained by the fact that chronic exposure to hyperglycemia with late diagnosis can ultimately lead to microvascular complications in patients with T2DM.

Finally, regarding macrovascular complications, no difference in the prevalence of coronary heart disease, peripheral artery disease, and stroke was observed between the two groups in this study. A negative correlation between GADA and CVD was also revealed in this results. Most studies have found that patients with LADA have lower central adiposity, a healthier lipid profile, and lower BP than patients with T2DM[11],[35]. In such patients, these factors collectively suggest a lower risk of macrovascular complications. This results matched those of the BOTNIA study that did not identify statistically significant differences between LADA and T2DM in coronary heart disease, stroke, overall cardiovascular morbidity, and mortality[47], similar results have been achieved by other independent studies[43], whereas the HUNT2 study showed that GADA-positive patients had a similar risk of cardiovascular disease and ischemic heart disease as GADA-negative patients with T2DM[48]. However, this is not fully explained by hyperglycemia, suggesting that the development of cardiovascular disease is based on a different pathophysiological mechanism. This hypothesis warrants further investigation of the potential pathways of autoimmune diabetes atherogenesis irrespective of the metabolic profile[49].


  Conclusion Top


This study showed 12.8% prevalence of positive diabetes-associated auto-antibodies namely GADA among patients with T2DM in the analyzed sample of the Egyptian population, which agrees with the already revealed findings concerning some other populations. In daily practice, this means that we can measure GADA in patients with early T2DM, and in the case of positive antibodies, an earlier start of insulin treatment is often needed. Future large-scale studies, including all types of auto-antibodies, will bring more light into the field of autoimmune diabetes. A personalized approach to medicine is most likely required to achieve optimal metabolic control and preserve β-cell function. This is associated with a lower risk of long-term diabetic complications and helps improve these patients' quality of life.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Brahmkshatriya PP, Mehta AA, Saboo BD, Goyal RK. Characteristics and prevalence of latent autoimmune diabetes in adults (LADA). ISRN Pharmacol 2012; 2012:580202.  Back to cited text no. 1
    
2.
Hegazi R, El-Gamal M, Abdel-Hady N, Hamdy O. Epidemiology of and risk factors for type 2 diabetes in Egypt. Ann Glob Health 2015; 81:814–820.  Back to cited text no. 2
    
3.
Tuomi T, Santoro N, Caprio S, Cai M, Weng J, Groop L. The many faces of diabetes: a disease with increasing heterogeneity. Lancet 2014; 383:1084–1094.  Back to cited text no. 3
    
4.
Leslie RD, Valeri C. Latent autoimmune diabetes in adults. Diabetes Voice 2003; 48:39–42.  Back to cited text no. 4
    
5.
American Diabetes Association. Classification and diagnosis of diabetes. Diabetes Care 2017; 40:S11–S24.  Back to cited text no. 5
    
6.
Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998; 15:539–553.  Back to cited text no. 6
    
7.
Appel SJ, Wadas TM, Rosenthal RS, Ovalle F. Latent autoimmune diabetes of adulthood (LADA): an often misdiagnosed type of diabetes mellitus. J Am Acad Nurse Pract 2009; 21:156–159.  Back to cited text no. 7
    
8.
Fourlanos S, Dotta F, Greenbaum CJ, Palmer JP, Rolandsson O, Colman PG, et al. Latent autoimmune diabetes in adults (LADA) should be less latent. Diabetologia 2005; 48:2206–2212.  Back to cited text no. 8
    
9.
Fourlanos S. A clinical screening tool identifies autoimmune diabetes in adults. Diabetes Care 2006; 29:970–975.  Back to cited text no. 9
    
10.
Niu X, Luo S, Li X, Xie Z, Xiang Y, Huang G, et al. Identification of a distinct phenotype of elderly latent autoimmune diabetes in adults: LADA China Study 8. Diabetes Metab Res Rev 2018; 35:e3068.  Back to cited text no. 10
    
11.
Hawa MI, Kolb H, Schloot N, Beyan H, Paschou SA, Buzzetti R, et al. Adult-onset autoimmune diabetes in Europe is prevalent with a broad clinical phenotype: Action LADA 7. Diabetes Care 2013; 36:908–913.  Back to cited text no. 11
    
12.
Buzzetti R, DiPietro S, Giaccari A, Petrone A, Locatelli M, Suraci C, et al. Non Insulin Requiring Autoimmune Diabetes Study Group. High titer of autoantibodies to GAD identifies a specific phenotype of adult-onset autoimmune diabetes. Diabetes Care 2007; 30:932–938.  Back to cited text no. 12
    
13.
International Diabetes Federation. IDF Diabetes Atlas. 6th edition. Belgium: International, Brussel. 2013.  Back to cited text no. 13
    
14.
Maddaloni E, Pozzilli P. SMART diabetes: the way to go (safe and multifactorial approach to reduce the risk for therapy in diabetes). Endocrine 2014; 46:3–5.  Back to cited text no. 14
    
15.
Maruyama T, Tanaka S, Shimada A, Funae O, Kasuga A, Kanatsuka A, et al. Insulin intervention in slowly progressive insulin-dependent (type 1) diabetes mellitus. J Clin Endocrinol Metab 2008; 93:2115–2121.  Back to cited text no. 15
    
16.
Laugesen E, Østergaard JA, Leslie RD. Danish Diabetes Academy Workshop and Workshop Speakers. Latent autoimmune diabetes of the adult: current knowledge and uncertainty. Diabet Med 2015; 32:843–852.  Back to cited text no. 16
    
17.
Radtke MA, Midthjell K, Nilsen TI, Grill V. Heterogeneity of patients with latent autoimmune diabetes in adults: linkage to autoimmunity is apparent only in those with perceived need for insulin treatment: results from the Nord-Trondelag Health (HUNT) study. Diabetes Care 2009; 32:245–250.  Back to cited text no. 17
    
18.
Turner R, Stratton I, Horton V, Manley S, Zimmet P, Mackay IR, et al. UKPDS 25: autoantibodies to islet-cell cytoplasm and glutamic acid decarboxylase for prediction of insulin requirement in type 2 diabetes. UK Prospective Diabetes Study Group. Lancet 1997; 350:1288–1293.  Back to cited text no. 18
    
19.
Tuomi T, Carlsson A, Li H, Isomaa B, Miettinen A, Nilsson A, et al. Clinical and genetic characteristics of type 2 diabetes with and without GAD antibodies. Diabetes 1999; 48:150–157.  Back to cited text no. 19
    
20.
Hawa MI, Buchan AP, Ola T, Wun CC, DeMicco DA, Bao W, et al. LADA and CARDS: a prospective study of clinical outcome in established adult-onset autoimmune diabetes. Diabetes Care 2014; 37:1643–1649.  Back to cited text no. 20
    
21.
Zaharieva ET, Velikova TV, Tsakova AD, Kamenov ZA. Prevalence of positive diabetes-associated autoantibodies among type 2 diabetes and related metabolic and inflammatory differences in a sample of the Bulgarian population. J Diabetes Res 2017; 2017:9016148.  Back to cited text no. 21
    
22.
Damanhouri LH, Dromey JA, Christie MR, Nasrat HA, Ardawi MS, Robins RA, et al. Autoantibodies to GAD and IA-2 in Saudi Arabian diabetic patients. Diabet Med 2005; 22:448–452.  Back to cited text no. 22
    
23.
Maioli M, Pes GM, Delitala G, Puddu L, Falorni A, Tolu F, et al. Number of autoantibodies and HLA genotype, more than high titers of glutamic acid decarboxylase autoantibodies, predict insulin dependence in latent autoimmune diabetes of adults. Eur J Endocrinol 2010; 163:541–549.  Back to cited text no. 23
    
24.
Zinman B, Kahn SE, Haffner SM, O'Neill MC, Heise MA, Freed MI. Phenotypic characteristics of GAD antibody-positive recently diagnosed patients with type 2 diabetes in North America and Europe. Diabetes 2004; 53:3193–3200.  Back to cited text no. 24
    
25.
Zhou Z, Xiang Y, Ji L, Jia W, Ning G, Huang G, et al. Frequency, immunogenetics, and clinical characteristics of latent autoimmune diabetes in China (LADA China study): a nationwide, multicenter, clinic-based cross-sectional study. Diabetes 2013; 62:543–550.  Back to cited text no. 25
    
26.
Takeda H, Kawasaki E, Shimizu I, Konoue E, Fujiyama M, Murao S, et al. Clinical, autoimmune, and genetic characteristics of adultonset diabetic patients with GAD autoantibodies in Japan (Ehime Study). Diabetes Care 2002; 25:995–1001.  Back to cited text no. 26
    
27.
Roh MO, Jung CH, Kim BY, Mok JO, Kim CH. The prevalence and characteristics of latent autoimmune diabetes in adults (LADA) and its relation with chronic complications in a clinical department of a university hospital in Korea. Acta Diabetol 2013; 50:129–134.  Back to cited text no. 27
    
28.
Temneanu OR, Trandafir LM, Purcarea MR. Type 2 diabetes mellitus in children and adolescents: a relatively new clinical problem within pediatric practice. J Med Life 2016; 3:235–239.  Back to cited text no. 28
    
29.
Schlosser M, Mueller PW, Torn C, Bonifacio E, Bingley PJ. Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies. Diabetologia 2010; 53:2611–2620.  Back to cited text no. 29
    
30.
Rasouli B, Grill V, Midthjell K, Ahlbom A, Andersson T, Carlsson S. Smoking is associated with reduced risk of autoimmune diabetes in adults contrasting with increased risk in overweight men with type 2 diabetes: a 22-year follow-up of the HUNT study. Diabetes Care 2003; 36:604–610.  Back to cited text no. 30
    
31.
Hampe CS, Hall TR, Agren A, Rolandsson O. Longitudinal changes in epitope recognition of autoantibodies against glutamate decarboxylase 65 (GAD65Ab) in prediabetic adults developing diabetes. Clin Exp Immunol 2007; 148:72–78.  Back to cited text no. 31
    
32.
Römkens TE, Kusters GC, Netea MG, Netten PM. Prevalence and clinical characteristics of insulin-treated, anti-gAd-positive, type 2 diabetic subjects in an outpatient clinical department of a dutch teaching hospital. J Med 2006; 64:114–118.  Back to cited text no. 32
    
33.
Groop LC, Bottazzo GF, Doniach D. Islet cell antibodies identify latent type 1 diabetes in patients aged 35–75 years at diagnosis. Diabetes 1986; 35:237–241.  Back to cited text no. 33
    
34.
Gambelunghe G, Forini F, Laureti S, Murdolo G, Toraldo G, Santeusanio F, et al. Increased risk for enocrine autoimmunity in Italian type 2 diabetic patients with GAD65 autoantibodies. Clin Endocrinol (Oxf) 2000; 52:563–573.  Back to cited text no. 34
    
35.
Maddaloni E, Lessan N, Al Tikriti A, Buzzetti R, Pozzilli P, Barakat MT. Latent autoimmune diabetes in adults in the United Arab Emirates: clinical features and factors related to insulin-requirement. PLoS One 2015; 10:e0131837.  Back to cited text no. 35
    
36.
Hjort R, Ahlqvist E, Carlsson PO, Grill V, Groop L, Martinell M, et al. Overweight, obesity and the risk of LADA: results from a Swedish case–control study and the Norwegian HUNT Study. Diabetologia 2018; 61:1333–1343.  Back to cited text no. 36
    
37.
Andersen CD, Bennet L, Nystrom L, Lindblad U, Lindholm E, Groop L, et al. Worse glycaemic control in LADA patients than in those with type 2 diabetes, despite a longer time on insulin therapy. Diabetologia 2013; 56:252–258.  Back to cited text no. 37
    
38.
Thunander M, Thorgeirsson H, Torn C, Petersson C, Landin- Olsson M. Beta-cell function and metabolic control in latent autoimmune diabetes in adults with early insulin versus conventional treatment: a 3-year follow-up. Eur J Endocrinol 2011; 164:239–245.  Back to cited text no. 38
    
39.
Genovese S, Bazzigaluppi E, Goncalves D, Ciucci A, Cavallo MG, Purrello F, et al. Clinical phenotype and beta-cell autoimmunity in Italian patients with adult-onset diabetes. Eur J Endocrinol 2006; 154:441–447.  Back to cited text no. 39
    
40.
Lundgren VM, Isomaa B, Lyssenko V, Laurila E, Korhonen P, Groop LC, et al. GAD antibody positivity predicts type 2 diabetes in an adult population. Diabetes 2010; 59:416–422.  Back to cited text no. 40
    
41.
Buzzetti R, Pozzilli P, Frederich R, Iqbal N, Hirshberg B. Saxagliptin improves glycaemic control and is well tolerated in adult diabetes patients with glutamic acid decarboxylase antibodies. Diabetologia 2013; 56:S390.  Back to cited text no. 41
    
42.
Johansen OE, Boehm BO, Grill V, Torjesen PA, Bhattacharya S, Patel S, et al. C-Peptide levels in latent autoimmune diabetes in adults treated with linagliptin versus glimepiride: exploratory results from a 2-year double-blind, randomized, controlled study. Diabetes Care 2014; 37:E11–E12.  Back to cited text no. 42
    
43.
Myhill P, Davis WA, Bruce DG, Mackay IR, Zimmet P, Davis TM. Chronic complications and mortality in community-based patients with latent autoimmune diabetes in adults: the Fremantle Diabetes Study. Diabet Med 2008; 25:1245–1250.  Back to cited text no. 43
    
44.
Lu J, Hou X, Zhang L, Hu C, Zhou J, Pang C, et al. Associations between clinical characteristics and chronic complications in latent autoimmune diabetes in adults and type 2 diabetes. Diabetes Metab Res Rev 2015; 31:411–420.  Back to cited text no. 44
    
45.
Wang C, Lu J, Lu W, Yu H, Jiang L, Li M, et al. Evaluating peripheral nerve function in asymptomatic patients with type 2 diabetes or latent autoimmune diabetes of adults (LADA): results from nerve conduction studies. J Diabetes Complications 2015; 29:265–269.  Back to cited text no. 45
    
46.
Baum P, Hermann W, Verlohren HJ, Wagner A, Lohmann T, Grahmann F. Diabetic neuropathy in patients with latent autoimmune diabetes of the adult (LADA) compared with patients with type 1 and type 2 diabetes. J Neurol 2003; 250:682–687.  Back to cited text no. 46
    
47.
Isomaa B, Almgren P, Henricsson M, Taskinen MR, Tuomi T, Groop L, et al. Chronic complications in patients with slowly progressing autoimmune type 1 diabetes (LADA). Diabetes Care 1999; 22:1347–1353.  Back to cited text no. 47
    
48.
Olsson L, Grill V, Midthjell K, Ahlbom A, Andersson T, Carlsson S. Mortality in adult-onset autoimmune diabetes is associated with poor glycemic control: results from the HUNT Study. Diabetes Care 2013; 36:3971–3978.  Back to cited text no. 48
    
49.
Lind M, Svensson AM, Rosengren A. Glycemic control and excess mortality in type 1 diabetes. N Engl J Med 2014; 371:1972–1982.  Back to cited text no. 49
    



 
 
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  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]



 

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